Insulin-Degrading Enzyme (IDE) (Internal Region) peptide (Biotin)
IDE
Reactivité: Humain
Hôte: Synthetic
BP, Imm
N° du produit ABIN5539423
Aperçu rapide pour Insulin-Degrading Enzyme (IDE) (Internal Region) peptide (Biotin) (ABIN5539423)
Antigène
IDE
(Insulin-Degrading Enzyme (IDE))
Origine
Humain
Source
Synthetic
Application
Blocking Peptide (BP), Immunogen (Imm)
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Protein Region
- Internal Region
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Purification/Conjugué
- This IDE peptide is labeled with Biotin.
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Séquence
- CQQYNFDRDN TE.
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Homologie
- Human, Dog
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Indications d'application
- When peptides are reconstituted in 200 μL water, the concentration would be also 0.5 mg/mL. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20 min. Then incubate and develop in parallel the two blots.Approx 120 kDa band observed in lysates of cell line K562 (calculated MW of 118 kDa according to NP_004960.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.3-1 μg/mL.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
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Buffer
- 100µg of Lyophilised peptide, formulation De-ionized (DI) water.
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Stock
- -20 °C
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Stockage commentaire
- Shipped at ambient temperature, store at -20°C.
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- IDE (Insulin-Degrading Enzyme (IDE))
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ID gène
- 3416
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NCBI Accession
- NP_004960, NP_001159418, NP_001309722, NP_001309723, NP_001309724, NP_001309726
Antigène
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